Adhesion of differentCandidaspp. to plastic: XTT formazan determinations
نویسندگان
چکیده
منابع مشابه
Hydrosoluble formazan XTT: its application to natural products drug discovery for Leishmania.
A colorimetric method for measuring the viability of Leishmania promastigotes is described that is based on the reduction of the tetrazolium salt, XTT, to a water-soluble formazan. Values obtained by the XTT method correlated well with parasite number (r=0.965) and with methods that rely upon the reduction of MTT or MTS (r=0.96 and 0.97, respectively). The IC(50) values obtained by XTT method w...
متن کاملComparative study of regular insulin adhesion to the walls of glass and plastic serum containers
Introduction:Due to the role of insulin in the control of blood sugar and its widespread use,it is very important to fine-tune the amount of insulin that should reach the patient so that the patient is not exposed to the complications of non-regulation of blood sugar. Research has shown that the choice of solution and serum containers is effective in the patient's access to insulin.Our goal is ...
متن کاملUsing the Xtt Approach
Practical development of the intelligent business applications for the Semantic Web requires advanced methods and tools for knowledge processing and representation. XML-based languages allow for knowledge specification, however they do not support inference which is the core activity. In the paper it is proposed to apply a new knowledge representation for expert systems, the eXtended Tabular Tr...
متن کاملLocalization of MTT formazan in lipid droplets. An alternative hypothesis about the nature of formazan granules and aggregates.
MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-dihphenyltetrazolium bromide) assay is a widely used method to assess cell viability and proliferation. MTT is readily taken up by cells and enzymatically reduced to formazan, a dark compound which accumulates in cytoplasmic granules. Formazan is later eliminated by the cell by a mechanisms often indicated as exocytosis, that produces characteristic needl...
متن کاملEffects of formocresol alone vs. formocresol with eugenol on macrophage adhesion to plastic surfaces.
PURPOSE The purpose of this study was to compare the in-vitro effects of a European-based formocresol formulation that incorporates eugenol with formocresol alone on the adhesion of macrophages to plastic surfaces. METHODS Macrophages were obtained from Wistar rats. The adherence capacity of macrophages to a plastic surface was determined. Assays were carried out in Eppendorf tubes incubated ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Medical Mycology
سال: 1996
ISSN: 1369-3786,1460-2709
DOI: 10.1080/02681219680000721